Pafah1b1^tm1Or
Targeted Allele Detail

Summary

• Symbol: Pafah1b1^tm1Or
• Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1; targeted mutation 1, Orly Reiner
• MGI ID: MGI:2387783
• Synonyms: sLIS1
• Gene: Pafah1b1 Location: Chr11:74564775-74615210 bp, - strand Genetic Position: Chr11, 45.76 cM, cytoband B3
• Alliance: Pafah1b1^tm1Or page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69631
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Flanking loxP sites and a neo cassette were introduced to the first ATG codon via homologous recombination. The ATG and neo cassette were removed by mating mutant animals to a PGK-cre line. The deletion results in translation from the second methionine and produces a shorter protein of 345 amino acids (instead of 409 amino acids). The mutant allele was confirmed by Western blot analysis of brain extracts from heterozygous animals. No homozygous mutant animals were born. (J:69631)

Disease models

Expression

In Mice Carrying this Mutation:

51 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pafah1b1 Mutation: 160 strains or lines available

References

• Original: J:69631 Cahana A, et al., Targeted mutagenesis of Lis1 disrupts cortical development and LIS1 homodimerization. Proc Natl Acad Sci U S A. 2001 May 22;98(11):6429-34
• All: 5 reference(s)