Tsix^tm1Jtl
Targeted Allele Detail

Summary

• Symbol: Tsix^tm1Jtl
• Name: X (inactive)-specific transcript, opposite strand; targeted mutation 1, Jeannie T Lee
• MGI ID: MGI:2387816
• Synonyms: Tsix^deltaCpG
• Gene: Tsix Location: ChrX:102475123-102528563 bp, + strand Genetic Position: ChrX, 46.13 cM
• Alliance: Tsix^tm1Jtl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57967
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette replaced 3.7 kb of sequence encompassing a CpG island, transcriptional start sites, and the putative promoter. RT-PCR and RNA FISH analyses of properly recombined ES cells showed an absence of transcript. (J:57967)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tsix Mutation: 57 strains or lines available

References

• Original: J:57967 Lee JT, et al., Targeted mutagenesis of Tsix leads to nonrandom X inactivation. Cell. 1999 Oct 1;99(1):47-57
• All: 5 reference(s)