Tsix^tm2Jtl
Targeted Allele Detail

Summary

• Symbol: Tsix^tm2Jtl
• Name: X (inactive)-specific transcript, opposite strand; targeted mutation 2, Jeannie T Lee
• MGI ID: MGI:2387818
• Synonyms: Tsix^deltaCpG
• Gene: Tsix Location: ChrX:102475123-102528563 bp, + strand Genetic Position: ChrX, 46.13 cM
• Alliance: Tsix^tm2Jtl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57967
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129 X (M. m. castaneus x 129)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette replaced 3.7 kb of sequence encompassing a CpG island, transcriptional start sites, and the putative promoter. Absence of a 129-specific MnlI restriction pattern indicated that the 129-derived X chromosome was targeted. Expression analysis showed a significant reduction in, if not a complete ablation of, transcription from the targeted allele, though transcript was shown to be produced from the normal X on the untargeted chromosome. Residual transcription was only detected by increased PCR cycles, though it was not clear if this weak transcription was due to minor and/or cryptic promoters or rather to overcylcing conditions. No residual transcription was detected by RNA FISH. (J:57967)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tsix Mutation: 57 strains or lines available

Notes

ES cell line = 16.7, a stable 40XX subclone of ES cell line EL16. 16.7 cells carry one X chromosome from 129 and the other from M. m. castaneus. Southern blot analysis showed the 129 chromosome to be targeted.

References

• Original: J:57967 Lee JT, et al., Targeted mutagenesis of Tsix leads to nonrandom X inactivation. Cell. 1999 Oct 1;99(1):47-57
• All: 1 reference(s)