Rad50^tm1Jpt
Targeted Allele Detail

Summary

• Symbol: Rad50^tm1Jpt
• Name: RAD50 double strand break repair protein; targeted mutation 1, John H J Petrini
• MGI ID: MGI:2387852
• Synonyms: m1, mrad50^Brdm1, Rad50^delta
• Gene: Rad50 Location: Chr11:53540346-53598146 bp, - strand Genetic Position: Chr11, 31.98 cM, cytoband A5-B1
• Alliance: Rad50^tm1Jpt page

Development of Rad50^tm1Jpt/Rad50^tm1Jpt embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56083
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The gene was disrupted by replacement of exon 1 with a PGK-neo cassette via homologous recombination. Homozygous mutant embryos were identified by PCR genotyping. (J:56083)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rad50 Mutation: 53 strains or lines available

References

• Original: J:56083 Luo G, et al., Disruption of mRad50 causes embryonic stem cell lethality, abnormal embryonic development, and sensitivity to ionizing radiation. Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7376-81
• All: 4 reference(s)