Rad50^tm2Jpt
Targeted Allele Detail

Summary

• Symbol: Rad50^tm2Jpt
• Name: RAD50 double strand break repair protein; targeted mutation 2, John H J Petrini
• MGI ID: MGI:2387857
• Synonyms: Rad50^K22M, Rad50^S
• Gene: Rad50 Location: Chr11:53540346-53598146 bp, - strand Genetic Position: Chr11, 31.98 cM, cytoband A5-B1
• Alliance: Rad50^tm2Jpt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78820
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: An engineered A to T mutation in exon 1 (c.65A>T) results in a lysine to methionine substitution at amino acid 22 in the encoded peptide (p.K22M). A floxed puromyin resistance gene cassette was inserted upstream of exon 1. The puro cassette was excised by crossing mutant animals to the cre-expressing line Tg(CMV-cre)1Nagy. (J:78820)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad50 Mutation: 53 strains or lines available

References

• Original: J:78820 Bender CF, et al., Cancer predisposition and hematopoietic failure in Rad50(S/S) mice. Genes Dev. 2002 Sep 1;16(17):2237-51
• All: 7 reference(s)