Sod1^tm1Dkd
Targeted Allele Detail

Summary

• Symbol: Sod1^tm1Dkd
• Name: superoxide dismutase 1, soluble; targeted mutation 1, Dipak K Das
• MGI ID: MGI:2387861
• Synonyms: Cu/ZnSOD-KO, Sod 1^-
• Gene: Sod1 Location: Chr16:90017650-90023221 bp, + strand Genetic Position: Chr16, 51.56 cM, cytoband B5-C3
• Alliance: Sod1^tm1Dkd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60470
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exon 5 and flanking intronic sequences with a neomycin resistance cassette via homologous recombination. The mutation deletes C-terminal amino acids 120-154 which constitute the structure and function of the active site channel. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot analysis of heart tissue. (J:60470)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sod1 Mutation: 40 strains or lines available

References

• Original: J:60470 Yoshida T, et al., Targeted disruption of the mouse Sod I gene makes the hearts vulnerable to ischemic reperfusion injury. Circ Res. 2000 Feb 18;86(3):264-9
• All: 2 reference(s)