Otp^tm1Tlu
Targeted Allele Detail

Summary

• Symbol: Otp^tm1Tlu
• Name: orthopedia homeobox; targeted mutation 1, Thomas Lufkin
• MGI ID: MGI:2387865
• Synonyms: Otp^lacZ
• Gene: Otp Location: Chr13:95012144-95021633 bp, + strand Genetic Position: Chr13, 49.22 cM
• Alliance: Otp^tm1Tlu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65759
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Sequence encoding the third helix of the homeodomain was disrupted by the insertion of a lacZ reporter cassette. The inserted cassette contained stop codons in all three reading frames, precluding the translation of the endogenous carboxy terminal, which is involved in transactivation. Comparisons with wild-type in situ hybridization results showed that the expression of lacZ was similar to the endogenous expression pattern. (J:65759)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otp Mutation: 17 strains or lines available

References

• Original: J:65759 Wang W, et al., The murine otp homeobox gene plays an essential role in the specification of neuronal cell lineages in the developing hypothalamus. Dev Biol. 2000 Nov 15;227(2):432-49
• All: 1 reference(s)