Tpm1^tm1Dfw
Targeted Allele Detail

Summary

• Symbol: Tpm1^tm1Dfw
• Name: tropomyosin 1, alpha; targeted mutation 1, David F Wieczorek
• MGI ID: MGI:2387893
• Synonyms: alphaTM1^-
• Gene: Tpm1 Location: Chr9:66929872-66956688 bp, - strand Genetic Position: Chr9, 36.27 cM
• Alliance: Tpm1^tm1Dfw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45833
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The striated muscle-specific exons 12 and 13 were replaced with an HPRT cassette in the reverse orientation. While the targeting construct was designed to knock-out the striated muscle isoform, the targeted allele is null. Interestingly, heterozygotes exhibit normal protein levels, despite a ~50% reduction in mRNA levels and absence of compensation by the striated muscle beta or the slow skeletal alpha isoforms, suggesting that a compensatory mechanism may operate at the translational level. (J:45833)

Expression

In Mice Carrying this Mutation:

18 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpm1 Mutation: 31 strains or lines available

References

• Original: J:45833 Rethinasamy P, et al., Molecular and physiological effects of alpha-tropomyosin ablation in the mouse [see comments]. Circ Res. 1998 Jan 9-23;82(1):116-23
• All: 2 reference(s)