H2-Ab1^b-tm1Hpl
Targeted Allele Detail

Summary

• Symbol: H2-Ab1^b-tm1Hpl
• Name: histocompatibility 2, class II antigen A, beta 1; targeted mutation 1, Hidde Ploegh
• MGI ID: MGI:2387946
• Synonyms: H2-Ab1^tm1Hpl, I-A^b-GFP, MHC II-EGFP, MHCII-EGFP, MHCIIGFP
• Gene: H2-Ab1 Location: Chr17:34482201-34488392 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: H2-Ab1^b-tm1Hpl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78940
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: This allele encodes an intact class II beta chain with an EGFP moiety fused to the carboxy terminal. The fusion protein was detected in whole spleen lysates, whereas free EGFP was not. An intronic floxed neo cassette upstream of the 3' untranslated region was included for selection. (J:78940)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any H2-Ab1 Mutation: 83 strains or lines available

Notes

J1 ES cells carry the b-haplotype of H2, which carries a mutation in the Ea promoter preventing the cell surface expression of the I-E complex.

References

• Original: J:78940 Boes M, et al., T-cell engagement of dendritic cells rapidly rearranges MHC class II transport. Nature. 2002 Aug 29;418(6901):983-8
• All: 32 reference(s)