Pou3f1^tm2.1Mejr
Targeted Allele Detail

Summary

• Symbol: Pou3f1^tm2.1Mejr
• Name: POU domain, class 3, transcription factor 1; targeted mutation 2.1, Dies Meijer
• MGI ID: MGI:2387953
• Synonyms: Oct-6^deltaSCE
• Gene: Pou3f1 Location: Chr4:124551439-124554448 bp, + strand Genetic Position: Chr4, 57.86 cM
• Alliance: Pou3f1^tm2.1Mejr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78939
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph, Modified regulatory region)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The floxed puromycin selection cassette was removed from Pou3f1^tm2Mejr by in vivo cre mediated excision, producing a hypomorphic allele lacking the 4.3 kb Schwann cell specific enhancer element. While protein was undetected by immunohistochemical analysis of Schwann cell nuclei isolated from homozygous mutant mice, low levels were detected by Western blot analysis. Protein expression was unaffected in the brainstem. (J:78939)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pou3f1 Mutation: 14 strains or lines available

References

• Original: J:78939 Ghazvini M, et al., A cell type-specific allele of the POU gene Oct-6 reveals Schwann cell autonomous function in nerve development and regeneration. EMBO J. 2002 Sep 2;21(17):4612-20
• All: 4 reference(s)