Eif2ak4tm1Drc
Targeted Allele Detail
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Symbol: |
Eif2ak4tm1Drc |
Name: |
eukaryotic translation initiation factor 2 alpha kinase 4; targeted mutation 1, Douglas R Cavener |
MGI ID: |
MGI:2387965 |
Synonyms: |
Gcn2-, Gcn2-Neo |
Gene: |
Eif2ak4 Location: Chr2:118219099-118305715 bp, + strand Genetic Position: Chr2, 59.4 cM, cytoband E5
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Alliance: |
Eif2ak4tm1Drc page
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Eif2ak4tm1Drc/Eif2ak4tm1Drc mice fed a leucine-devoid diet appear scruffy and lethargic
Show the 1 phenotype image(s) involving this allele.
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Germline Transmission: |
Earliest citation of germline transmission:
J:78945
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Parent Cell Line: |
TL1/TL-1 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: The majority of exon 12, which encodes the kinase subdomains 2 through 4 common to the 3 known isoforms (alpha, beta, and gamma), was replaced by a neomycin selection cassette inserted by homologous recombination. While, RT-PCR analysis showed the presence of an aberrant splice product, sequence analysis showed it generated a frameshift mutation in the open reading frame. Western blot analysis verified the absence of protein.
(J:78945)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Eif2ak4 Mutation: |
89 strains or lines available
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Original: |
J:78945 Zhang P, et al., The GCN2 eIF2alpha kinase is required for adaptation to amino acid deprivation in mice. Mol Cell Biol. 2002 Oct;22(19):6681-8 |
All: |
18 reference(s) |
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