About   Help   FAQ
Eif2ak4tm1Drc
Targeted Allele Detail
Summary
Symbol: Eif2ak4tm1Drc
Name: eukaryotic translation initiation factor 2 alpha kinase 4; targeted mutation 1, Douglas R Cavener
MGI ID: MGI:2387965
Synonyms: Gcn2-, Gcn2-Neo
Gene: Eif2ak4  Location: Chr2:118219099-118305715 bp, + strand  Genetic Position: Chr2, 59.4 cM, cytoband E5
Alliance: Eif2ak4tm1Drc page
Eif2ak4tm1Drc/Eif2ak4tm1Drc mice fed a leucine-devoid diet appear scruffy and lethargic

Show the 1 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:78945
Parent Cell Line:  TL1/TL-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe majority of exon 12, which encodes the kinase subdomains 2 through 4 common to the 3 known isoforms (alpha, beta, and gamma), was replaced by a neomycin selection cassette inserted by homologous recombination. While, RT-PCR analysis showed the presence of an aberrant splice product, sequence analysis showed it generated a frameshift mutation in the open reading frame. Western blot analysis verified the absence of protein. (J:78945)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Eif2ak4 Mutation:  89 strains or lines available
References
Original:  J:78945 Zhang P, et al., The GCN2 eIF2alpha kinase is required for adaptation to amino acid deprivation in mice. Mol Cell Biol. 2002 Oct;22(19):6681-8
All:  18 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/29/2024
MGI 6.24
The Jackson Laboratory