Ptger1^tm1Jdm
Targeted Allele Detail

Summary

• Symbol: Ptger1^tm1Jdm
• Name: prostaglandin E receptor 1 (subtype EP1); targeted mutation 1, John D McNeish
• MGI ID: MGI:2387971
• Synonyms: EP1^-
• Gene: Ptger1 Location: Chr8:84393307-84399382 bp, + strand Genetic Position: Chr8, 40.22 cM
• Alliance: Ptger1^tm1Jdm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79387
• Parent Cell Line: DBA-252 (ES Cell)
• Strain of Origin: DBA/1LacJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: 671 bp of coding sequence were replaced by the insertion of a neomycin selection cassette into exon 2. Transcript was undetected in liver, uterus, and kidney tissue by RT-PCR analysis and in situ hybridization of kidney tissue. Western blot analysis of Prkcl1 protein expression indicated that the targeting vector did not significantly disrupt the the Prkcl1 locus, which putatively overlaps the Ptger1 locus. (J:79387)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptger1 Mutation: 22 strains or lines available

References

• Original: J:79387 Stock JL, et al., The prostaglandin E2 EP1 receptor mediates pain perception and regulates blood pressure. J Clin Invest. 2001 Feb;107(3):325-31
• All: 11 reference(s)