Smcp^tm1Wen
Targeted Allele Detail

Summary

• Symbol: Smcp^tm1Wen
• Name: sperm mitochondria-associated cysteine-rich protein; targeted mutation 1, Wolfgang Engel
• MGI ID: MGI:2387973
• Synonyms: Smcp^-
• Gene: Smcp Location: Chr3:92491174-92496304 bp, - strand Genetic Position: Chr3, 40.14 cM
• Alliance: Smcp^tm1Wen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78930
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: Exon 2 was replaced by a neomycin selection cassette inserted by homologous recombination. Normal transcript was undetected in homozygous mutant mice by Northern blot analysis of testicular RNA. Western blot analysis showed that only the neomycin protein, and not the normal protein, was produced from the disrupted allele. (J:78930)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smcp Mutation: 15 strains or lines available

References

• Original: J:78930 Nayernia K, et al., Asthenozoospermia in mice with targeted deletion of the sperm mitochondrion-associated cysteine-rich protein (Smcp) gene. Mol Cell Biol. 2002 May;22(9):3046-52
• All: 3 reference(s)