Smarca2^tm1Mya
Targeted Allele Detail

Summary

• Symbol: Smarca2^tm1Mya
• Name: SWI/SNF related BAF chromatin remodeling complex subunit ATPase 2; targeted mutation 1, Moshe Yaniv
• MGI ID: MGI:2387988
• Synonyms: BRM^-
• Gene: Smarca2 Location: Chr19:26582578-26755721 bp, + strand Genetic Position: Chr19, 21.17 cM, cytoband C1
• Alliance: Smarca2^tm1Mya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51536
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The majority of the exon encoding amino acids 120-176 was replaced with a neomycin resistance cassette via homologous recombination. RT-PCR analysis of liver RNA from homozygous mutant animals revealed a faint truncated transcript resulting from an alternative splice product which skips the disrupted exon. Western blot analysis of brain nuclear extracts detected a shortened protein product expressed at less than 1% the amount of wild-type protein. Co-immunoprecipitation experiments showed that the mutant protein lacked functional capabilities. (J:51536)

Expression

In Mice Carrying this Mutation:	64 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Smarca2^tm1Mya

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smarca2 Mutation: 90 strains or lines available

References

• Original: J:51536 Reyes JC, et al., Altered control of cellular proliferation in the absence of mammalian brahma (SNF2alpha). EMBO J. 1998 Dec 1;17(23):6979-91
• All: 18 reference(s)