Smarca2tm1Mya
Targeted Allele Detail
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Symbol: |
Smarca2tm1Mya |
Name: |
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; targeted mutation 1, Moshe Yaniv |
MGI ID: |
MGI:2387988 |
Synonyms: |
BRM- |
Gene: |
Smarca2 Location: Chr19:26582578-26755721 bp, + strand Genetic Position: Chr19, 21.17 cM, cytoband C1
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Alliance: |
Smarca2tm1Mya page
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Germline Transmission: |
Earliest citation of germline transmission:
J:51536
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Parent Cell Line: |
CK35 (ES Cell)
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Strain of Origin: |
129S2/SvPas
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: The majority of the exon encoding amino acids 120-176 was replaced with a neomycin resistance cassette via homologous recombination. RT-PCR analysis of liver RNA from homozygous mutant animals revealed a faint truncated transcript resulting from an alternative splice product which skips the disrupted exon. Western blot analysis of brain nuclear extracts detected a shortened protein product expressed at less than 1% the amount of wild-type protein. Co-immunoprecipitation experiments showed that the mutant protein lacked functional capabilities.
(J:51536)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Smarca2 Mutation: |
90 strains or lines available
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Original: |
J:51536 Reyes JC, et al., Altered control of cellular proliferation in the absence of mammalian brahma (SNF2alpha). EMBO J. 1998 Dec 1;17(23):6979-91 |
All: |
18 reference(s) |
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