Mbd2^tm1Bh
Targeted Allele Detail

Summary

• Symbol: Mbd2^tm1Bh
• Name: methyl-CpG binding domain protein 2; targeted mutation 1, Brian Hendrich
• MGI ID: MGI:2388002
• Synonyms: Mbd2-, Mbd2^tm1.1Brd
• Gene: Mbd2 Location: Chr18:70701260-70759202 bp, + strand Genetic Position: Chr18, 44.54 cM
• Alliance: Mbd2^tm1Bh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68356
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 was deleted and replaced with a promoter-less beta-geo cassette via homologous recombination. Northern blot confirmed the absence of normal transcript in homozygous mutant animals. RT-PCR analysis using primers to exon 1 and exon 3 detected a faint mutant read-through product. Western blot analysis of spleen and liver nuclear extracts from homozygous animals detected a small mutant protein presumed to be the translation product from exon 1, and would lack any functional domains. (J:68356)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Mbd2^tm1Bh

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mbd2 Mutation: 21 strains or lines available

References

• Original: J:68356 Hendrich B, et al., Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development. Genes Dev. 2001 Mar 15;15(6):710-23
• All: 21 reference(s)