Nodal^tm3Rob
Targeted Allele Detail

Summary

• Symbol: Nodal^tm3Rob
• Name: nodal growth differentiation factor; targeted mutation 3, Elizabeth J Robertson
• MGI ID: MGI:2388004
• Synonyms: Nodal^delta, Nodal^deltanode
• Gene: Nodal Location: Chr10:61253751-61261117 bp, + strand Genetic Position: Chr10, 32.21 cM
• Alliance: Nodal^tm3Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79098
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Expression of the endogenous gene was reduced by the deletion of a 2.7 kb enhancer region. The targeting vector contained a floxed hygromycin cassette, which was excised in vitro via the expression of cre recombinase. In situ hybridization showed normal expression in early gastrulation stages, with attenuated expression begining during the early somite stages. Robust expression was observed in the left lateral plate mesoderm. (J:79098)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nodal Mutation: 42 strains or lines available

References

• Original: J:79098 Brennan J, et al., Nodal activity in the node governs left-right asymmetry. Genes Dev. 2002 Sep 15;16(18):2339-44
• All: 2 reference(s)