Mbd3^tm1Bh
Targeted Allele Detail

Summary

• Symbol: Mbd3^tm1Bh
• Name: methyl-CpG binding domain protein 3; targeted mutation 1, Brian Hendrich
• MGI ID: MGI:2388006
• Synonyms: Mbd3(-)
• Gene: Mbd3 Location: Chr10:80228373-80235365 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Mbd3^tm1Bh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68356
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2-7 were replaced by an internal ribosomal entry site (IRES) followed by a betageo (beta-galactosidase/neomycin resistance) cassette via homologous recombination so that beta-galactosidase is expressed from the endogenous promoter. Homozygous mutant embryos were observed up to day E8.5. (J:68356, J:167984)

Expression

In Mice Carrying this Mutation:

83 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mbd3 Mutation: 58 strains or lines available

References

• Original: J:68356 Hendrich B, et al., Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development. Genes Dev. 2001 Mar 15;15(6):710-23
• All: 4 reference(s)