Ambp^tm1Kkm
Targeted Allele Detail

Summary

• Symbol: Ambp^tm1Kkm
• Name: alpha 1 microglobulin/bikunin precursor; targeted mutation 1, Koji Kimata
• MGI ID: MGI:2388018
• Synonyms: Bik^-, IaI^-
• Gene: Ambp Location: Chr4:63061512-63072409 bp, - strand Genetic Position: Chr4, 33.96 cM, cytoband C1-C3
• Alliance: Ambp^tm1Kkm page

Impaired cumulus expansion and oocyte fertilization in Ambp^tm1Kkm/Ambp^tm1Kkm female mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68027
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo-derived cassette replaced exon 7 and the downstream intron via homologous recombination. The neo cassette introduces translation termination codons before serine 7 of the bikunin protein. The alpha 1 microglobulin sequence, which is translated as a fusion protein with bikunin, remains unchanged. (J:68027)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available
• Carrying any Ambp Mutation: 25 strains or lines available

References

• Original: J:68027 Zhuo L, et al., Defect in SHAP-hyaluronan complex causes severe female infertility. A study by inactivation of the bikunin gene in mice. J Biol Chem. 2001 Mar 16;276(11):7693-6
• All: 7 reference(s)