Raf1^tm1Bacc
Targeted Allele Detail

Summary

• Symbol: Raf1^tm1Bacc
• Name: v-raf-leukemia viral oncogene 1; targeted mutation 1, Manuela Baccarini
• MGI ID: MGI:2388026
• Synonyms: c-raf-1^-, Raf-1^-
• Gene: Raf1 Location: Chr6:115595530-115653596 bp, - strand Genetic Position: Chr6, 53.62 cM, cytoband C3
• Alliance: Raf1^tm1Bacc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69056
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 3 was flanked with loxP sites by insertion of a floxed TK-neo cassette and a single loxP site into the upstream and downstream introns, respectively. Exon 3 was removed by transient expression of cre recombinase in correctly targeted ES cells. Absence of gene expression was confirmed by Western blot analysis of mouse embryonic fibroblasts (MEFs) derived from homozygous mutant embryos. Immune complex kinase assays showed a lack of enzyme activity in MEFs from homozygous mutants. (J:69056)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Raf1 Mutation: 117 strains or lines available

References

• Original: J:69056 Mikula M, et al., Embryonic lethality and fetal liver apoptosis in mice lacking the c-raf-1 gene. EMBO J. 2001 Apr 17;20(8):1952-62
• All: 6 reference(s)