Smarcb1^{Gt(OST32815)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Smarcb1^{Gt(OST32815)Lex}
• Name: SWI/SNF related BAF chromatin remodeling complex subunit B1; gene trap OST32815, Lexicon Genetics
• MGI ID: MGI:2388033
• Synonyms: Ini1-, Ini1ⁱⁿ³, Smarcb1^{Gt(VICTR20)19Lex}
• Gene: Smarcb1 Location: Chr10:75732603-75757448 bp, - strand Genetic Position: Chr10, 38.61 cM
• Alliance: Smarcb1^{Gt(OST32815)Lex} page

Mutation origin

• Mutant Cell Line: OST32815
• Germline Transmission: Earliest citation of germline transmission: J:69009
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene trap vector consisting of LTR sites flanking a beta-geo cassette and a PGK-puro cassette integrated into intron 3 of the gene. A splice acceptor site in Beta-geo results in a lacZ fusion protein and a splice donor site at the end of PGK-puro introduces multiple stop codons into the transcribed gene. (J:69009)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST32815	CG494133 ; (MGI Seq Detail)	3' RACE	Chr10:75741879-75747489 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  5 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smarcb1 Mutation: 22 strains or lines available

References

• Original: J:69009 Guidi CJ, et al., Disruption of ini1 leads to peri-implantation lethality and tumorigenesis in mice. Mol Cell Biol. 2001 May 15;21(10):3598-603
• All: 5 reference(s)