Dlg2^tm1Dsb
Targeted Allele Detail

Summary

• Symbol: Dlg2^tm1Dsb
• Name: discs large MAGUK scaffold protein 2; targeted mutation 1, David S Bredt
• MGI ID: MGI:2388036
• Synonyms: Dlg2^deltaE9, PSD93-
• Gene: Dlg2 Location: Chr7:90125880-92098455 bp, + strand Genetic Position: Chr7, 51.07 cM
• Alliance: Dlg2^tm1Dsb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68924
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Hypomorph, Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: The exon encoding the second PDZ domain was replaced with a TK-neo cassette via homologous recombination. Northern blot analysis of brain RNA from homozygous mutant animals detected a faint mutant transcript of higher mobility, presumably an alternative splice product that skips the deleted exon. However, Western blot analysis of brain proteins using antibodies directed against a region N-terminal to the deleted exon did not detect protein product.
  Exon 9 is deleted. In knockout mice, the longer Dlg2gamma and DLG2alpha1 are not expressed in bone-marrow derived plasmacytoid dendritic cells. However, a shorter Dlg2n isoform is expressed in bone-marrow derived plasmacytoid dendritic cells and in brain (J:262606). (J:68924, J:262606)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dlg2 Mutation: 62 strains or lines available

References

• Original: J:68924 McGee AW, et al., PSD-93 knock-out mice reveal that neuronal MAGUKs are not required for development or function of parallel fiber synapses in cerebellum. J Neurosci. 2001 May 1;21(9):3085-91
• All: 22 reference(s)