Pik3cd^tm1Tnr
Targeted Allele Detail

Summary

• Symbol: Pik3cd^tm1Tnr
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta; targeted mutation 1, Martin Turner
• MGI ID: MGI:2388047
• Synonyms: p110delta-, Pik3cd^tm1Trn
• Gene: Pik3cd Location: Chr4:149733625-149787023 bp, - strand Genetic Position: Chr4, 80.15 cM
• Alliance: Pik3cd^tm1Tnr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79133
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 1 through 9, encoding the first 490 amino acids, were deleted by homologous recombination of floxed selection genes followed by cre mediated excision in male germ cells. Targeting was done in PC3 ES cells which are homozygous for a protamine-cre transgene, Tg(Prm-cre)70Og, which expresses Cre recombinase only in halpoid spermatids. Normal protein was undetected by Western blot analysis of thymocytes obtained from homozygous mutant mice. (J:79133)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pik3cd Mutation: 45 strains or lines available

References

• Original: J:79133 Clayton E, et al., A crucial role for the p110delta subunit of phosphatidylinositol 3-kinase in B cell development and activation. J Exp Med. 2002 Sep 16;196(6):753-63
• All: 22 reference(s)