Tulp3^tm1Jng
Targeted Allele Detail

Summary

• Symbol: Tulp3^tm1Jng
• Name: TUB like protein 3; targeted mutation 1, Jurgen Naggert
• MGI ID: MGI:2388066
• Synonyms: Tulp3^-
• Gene: Tulp3 Location: Chr6:128298124-128332814 bp, - strand Genetic Position: Chr6, 62.96 cM
• Alliance: Tulp3^tm1Jng page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70060
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of exon 5 and exons 6-9 were removed and replaced with an in-frame GFP-neo construct via homologous recombination. The targeted mutation results in deletion of the C-terminus and part of the N-terminal region of the protein, and creates a fusion protein with GFP. Homozygous mutant embryos were identified by PCR genotype analysis. (J:70060)

Expression

In Mice Carrying this Mutation:	10 assay results
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tulp3 Mutation: 54 strains or lines available

References

• Original: J:70060 Ikeda A, et al., Neural tube defects and neuroepithelial cell death in Tulp3 knockout mice. Hum Mol Genet. 2001 Jun 1;10(12):1325-34
• All: 4 reference(s)