Slc22a3^tm1Dpb
Targeted Allele Detail

Summary

• Symbol: Slc22a3^tm1Dpb
• Name: solute carrier family 22 (organic cation transporter), member 3; targeted mutation 1, Denise P Barlow
• MGI ID: MGI:2388117
• Synonyms: Oct3-, Orct3-deficient
• Gene: Slc22a3 Location: Chr17:12638859-12726591 bp, - strand Genetic Position: Chr17, 8.52 cM
• Alliance: Slc22a3^tm1Dpb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69978
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 and flanking intronic sequences were deleted and replaced with a floxed PGK-neo cassette via homologous recombination. Transient expression of cre recombinase in correctly targeted cells removed the selection cassette leaving behind a single loxP site in place of exon 1. Gene expression was absent in the heart, brain, and skeletal muscle of homozygous mutant animals as determined by Northern blot analysis. However, an aberrant transcript was detected in placentas from homozygous mutant embryos. RT-PCR analysis revealed the mutant transcript results from alternative splicing of intron 2 to exon 3 and does not translate a functional protein product. (J:69978)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc22a3 Mutation: 29 strains or lines available

References

• Original: J:69978 Zwart R, et al., Impaired activity of the extraneuronal monoamine transporter system known as uptake-2 in Orct3/Slc22a3-deficient mice. Mol Cell Biol. 2001 Jul;21(13):4188-96
• All: 13 reference(s)