Mnat1^tm1Tpm
Targeted Allele Detail

Summary

• Symbol: Mnat1^tm1Tpm
• Name: menage a trois 1; targeted mutation 1, Tomi P Makela
• MGI ID: MGI:2388119
• Synonyms: Mat1(-)
• Gene: Mnat1 Location: Chr12:73170491-73320762 bp, + strand Genetic Position: Chr12, 30.37 cM, cytoband C3
• Alliance: Mnat1^tm1Tpm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69926
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An exon encoding 51 N-terminal amino acids comprising most of the RING finger domain was deleted and replaced with a PGK-neo cassette via homologous recombination. Western blot analysis of correctly targeted ES cells showed no detectable protein expression. Homozygous mutant embryos were observed at day E4.0 by PCR genotype analysis. (J:69926)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mnat1 Mutation: 26 strains or lines available

Notes

Homozygous mutant embryos are inviable and die shortly after implantation.

References

• Original: J:69926 Rossi DJ, et al., Inability to enter S phase and defective RNA polymerase II CTD phosphorylation in mice lacking Mat1. EMBO J. 2001 Jun 1;20(11):2844-56
• All: 2 reference(s)