Stam^tm1Sug
Targeted Allele Detail

Summary

• Symbol: Stam^tm1Sug
• Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 1; targeted mutation 1, Kazuo Sugamura
• MGI ID: MGI:2388123
• Synonyms: STAM1^-/-
• Gene: Stam Location: Chr2:14078912-14152351 bp, + strand Genetic Position: Chr2, 10.36 cM, cytoband A2-B
• Alliance: Stam^tm1Sug page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69504
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exons 3 and 4 encoding amino acids 44-99 with a neomycin resistance cassette. RT-PCR analysis of splenocyte RNA from homozygous mutant animals detected a mutant transcript with a frameshift and nonsense codon in exon 5. Western blot analysis of neocortex lysates and immunoprecipitation immunoblot of activated T cells from homozygous mutant animals did not detect a protein product. (J:69504)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Stam Mutation: 43 strains or lines available

References

• Original: J:69504 Yamada M, et al., Loss of hippocampal CA3 pyramidal neurons in mice lacking STAM1. Mol Cell Biol. 2001 Jun;21(11):3807-19
• All: 2 reference(s)