Top3b^tm1Jcw
Targeted Allele Detail

Summary

• Symbol: Top3b^tm1Jcw
• Name: topoisomerase (DNA) III beta; targeted mutation 1, James C Wang
• MGI ID: MGI:2388157
• Synonyms: top3beta^-/-
• Gene: Top3b Location: Chr16:16688600-16710854 bp, + strand Genetic Position: Chr16, 10.46 cM, cytoband B1
• Alliance: Top3b^tm1Jcw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69405
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A region of the gene spanning nucleotides 777 to 1490 of the coding sequence was replaced with a PGK-neo cassette. This region includes the active site tyrosine 336. Gene expression was undetectable by RT-PCR analysis of liver mRNA from homozygous mutant animals. (J:69405)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Top3b Mutation: 47 strains or lines available

References

• Original: J:69405 Kwan KY, et al., Mice lacking DNA topoisomerase IIIbeta develop to maturity but show a reduced mean lifespan. Proc Natl Acad Sci U S A. 2001 May 8;98(10):5717-21
• All: 5 reference(s)