Ascl1^{tm1(Neurog2)Fgu}
Targeted Allele Detail

Summary

• Symbol: Ascl1^{tm1(Neurog2)Fgu}
• Name: achaete-scute family bHLH transcription factor 1; targeted mutation 1, Francois Guillemot
• MGI ID: MGI:2388165
• Synonyms: Ascl1^Ngn2KI, Mash1^KINgn2
• Gene: Ascl1 Location: Chr10:87326681-87329522 bp, - strand Genetic Position: Chr10, 43.64 cM
• Alliance: Ascl1^{tm1(Neurog2)Fgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74525
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Ascl1^{tm1(Neurog2)Fgu} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Neurog2

• Mutation details: The entire coding region of the endogenous gene was replaced with that of Neurog2. The 5' and 3' untranslated regions of the endogenous gene were left intact, allowing Neurog2 to be expressed in the Ascl1 domain. A single loxP site was left in an intron following the inserted Neurog2 coding region. (J:74525)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Ascl1^tm1(Neurog2)Fgu/Ascl1^tm1(Neurog2)Fgu	involves: 129S1/Sv * 129X1/SvJ
ht2	Ascl1^tm1(Neurog2)Fgu/Ascl1^+	involves: 129S1/Sv * 129X1/SvJ

Phenotypes:

Affected Systems	hm1	ht2
show or hide all annotated terms
cellular	√
abnormal neuron apoptosis	√
mortality/aging	√
neonatal lethality, complete penetrance	√
nervous system	√	√
abnormal neuron apoptosis	√
abnormal locus ceruleus morphology	√
abnormal dorsal interneuron 2 morphology		√
abnormal dorsal interneuron 3 morphology		√
abnormal dorsal interneuron LA morphology	√
abnormal ventral interneuron 2 morphology	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ascl1 Mutation: 28 strains or lines available

References

• Original: J:74525 Parras CM, et al., Divergent functions of the proneural genes Mash1 and Ngn2 in the specification of neuronal subtype identity. Genes Dev. 2002 Feb 1;16(3):324-38
• All: 13 reference(s)