Dab1^tm2Cpr
Targeted Allele Detail

Summary

• Symbol: Dab1^tm2Cpr
• Name: disabled 1; targeted mutation 2, Jonathan A Cooper
• MGI ID: MGI:2388292
• Synonyms: Dab1-, dab1-5Fki
• Gene: Dab1 Location: Chr4:103476556-104602041 bp, + strand Genetic Position: Chr4, 47.79 cM
• Alliance: Dab1^tm2Cpr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79475
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: To generate an allele lacking phosphorylation sites, the exon encoding amino acids 23 through 69 was replaced with a construct containing a splice acceptor and a modified partial cDNA encoding the p80 splice form. The cDNA was modified to encode phenylalanines in place of the 5 tyrosines residues (185, 198, 200, 220, 232) that are potential phosphorylation sites. Phosphorylation levels were undetectable by phosphopeptide analysis of protein produced by the targeted allele in vitro. (J:79475)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dab1 Mutation: 79 strains or lines available

References

• Original: J:79475 Howell BW, et al., Dab1 tyrosine phosphorylation sites relay positional signals during mouse brain development. Curr Biol. 2000 Jul 27-Aug 10;10(15):877-85
• All: 10 reference(s)