Hmox1^tm1Poss
Targeted Allele Detail

Summary

• Symbol: Hmox1^tm1Poss
• Name: heme oxygenase 1; targeted mutation 1, Kenneth D Poss
• MGI ID: MGI:2388322
• Synonyms: Hmox1^-, HO-1^-, HO1^-
• Gene: Hmox1 Location: Chr8:75820249-75827217 bp, + strand Genetic Position: Chr8, 35.59 cM, cytoband C1
• Alliance: Hmox1^tm1Poss page

Iron-loading of Hmox1^tm1Poss/Hmox1^tm1Poss tissues.

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79254
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 3.7 kb fragment encompassing exons 3, 4, and a portion of 5 was replaced with a neomycin selection cassette. The deleted region consisted of approximately 85% of the coding region (226 residues). A low level of aberrantly spliced transcript was identified in homozygous mutant mice by Northern blot analysis of total splenic RNA. (J:79254)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Hmox1^tm1Poss/Hmox1^tm1Poss	involves: 129 * 129S2/SvPas * C57BL/6J
hm2  Disease Model	Hmox1^tm1Poss/Hmox1^tm1Poss	involves: 129S2/SvPas * C57BL/6
cx3	Hmox1^tm1Poss/Hmox1^tm1Poss Slc48a1^em1Ih/Slc48a1^em1Ih	involves: 129 * 129S2/SvPas * C57BL/6J
cx4	Hmox1^tm1Poss/Hmox1^+ Slc48a1^em1Ih/Slc48a1^em1Ih	involves: 129 * 129S2/SvPas * C57BL/6J
cx5	Hmox1^tm1Poss/Hmox1^tm1Poss Spic^tm2Kmm/Spic^tm2Kmm	involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6 * FVB/N

Phenotypes:

Affected Systems	hm1	hm2		cx3	cx4	cx5
show or hide all annotated terms Sex:
behavior/neurological		√	√
poor grooming		√	√
decreased locomotor activity		√	√
cellular		√	√
increased renal tubule apoptosis		√	√
oxidative stress		√	√
endocrine/exocrine glands			√
small testis			√
growth/size/body		√	√
decreased body size		√	√
cachexia		√	√
enlarged spleen		√	√
spleen hyperplasia		√	√
hematopoietic system	√	√	√	√	√	√
enlarged spleen		√	√
spleen hyperplasia		√	√
extramedullary hematopoiesis		√	√
anemia	√			√
microcytic anemia		√	√
impaired hematopoiesis					√
increased erythroid progenitor cell number					√
decreased erythrocyte cell number		√	√
decreased hematocrit		√	√
decreased hemoglobin content		√	√
decreased mean corpuscular volume		√	√
anisocytosis		√	√
abnormal leukocyte cell number		√	√
decreased macrophage cell number	√			√	√	√
abnormal splenic cell ratio		√	√
homeostasis/metabolism		√	√
increased blood urea nitrogen level		√	√
increased circulating ferritin level		√	√
abnormal iron homeostasis		√	√
decreased circulating iron level		√	√
immune system	√	√	√	√	√	√
enlarged spleen		√	√
spleen hyperplasia		√	√
abnormal leukocyte cell number		√	√
decreased macrophage cell number	√			√	√	√
abnormal splenic cell ratio		√	√
abnormal lymph node cell ratio		√	√
enlarged lymph nodes		√	√
chronic inflammation		√	√
liver inflammation		√	√
glomerulonephritis		√	√
lung inflammation		√	√
liver/biliary system		√	√
liver inflammation		√	√
liver fibrosis		√	√
mortality/aging	√	√	√	√	√
premature death		√	√
postnatal lethality, incomplete penetrance		√	√
prenatal lethality, incomplete penetrance	√			√	√
renal/urinary system		√	√
increased renal tubule apoptosis		√	√
glomerulonephritis		√	√
glomerulosclerosis		√	√
glomerular crescent		√	√
proximal convoluted tubule brush border loss		√	√
renal tubular necrosis		√	√
renal cast		√	√
kidney failure		√	√
reproductive system		√	√
small testis			√
infertility		√	√
respiratory system		√	√
lung inflammation		√	√

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Key:

√

disease model

expected model not found

Models:

Human Diseases	hm2
hemochromatosis IDs hemochromatosis       DOID:2352 ICD10CM:E83.11 MESH:D006432 NCI:C82892 OMIM:231100 OMIM:PS235200 ORDO:139498 UMLS_CUI:C0018995 Close	√

Expression

In Mice Carrying this Mutation:	48 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hmox1 Mutation: 35 strains or lines available

References

• Original: J:79254 Poss KD, et al., Heme oxygenase 1 is required for mammalian iron reutilization. Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10919-24
• All: 62 reference(s)