Tcra^tm1Cgn
Targeted Allele Detail

Summary

• Symbol: Tcra^tm1Cgn
• Name: T cell receptor alpha chain; targeted mutation 1,University of Cologne
• MGI ID: MGI:2388328
• Synonyms: Calpha^fl, Trac^fl
• Gene: Tcra Location: Chr14:52665424-54461655 bp, + strand Genetic Position: Chr14, cytoband D1-D2

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79298
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A loxP site was inserted upstream of Tcra-C element C1, and a loxP flanked neo was inserted between elements C3 and C4 via homologous recombination. Transient transfection of targeted ES cells removed the neo cassette leaving the conditional Calpha allele with C1, C2 and C3 flanked by loxP sites. (J:79298)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcra Mutation: 101 strains or lines available

References

• Original: J:79298 Polic B, et al., How alpha beta T cells deal with induced TCR alpha ablation. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8744-9
• All: 10 reference(s)