Camk4^tm2Arm
Targeted Allele Detail

Summary

• Symbol: Camk4^tm2Arm
• Name: calcium/calmodulin-dependent protein kinase IV; targeted mutation 2, Anthony R Means
• MGI ID: MGI:2388343
• Synonyms: CaSKO
• Gene: Camk4 Location: Chr18:33067984-33324281 bp, + strand Genetic Position: Chr18, 18.15 cM
• Alliance: Camk4^tm2Arm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70856
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The calspermin proximal promoter and testis-specific exon were replaced with a floxed TK-neo cassette via homologous recombination. Transient expression of cre recombinase in correctly targeted ES cells removed the selection cassette leaving behind a single loxP site. The gene targeting strategy is designed to specifically delete calspermin expression but not Camk4 kinase expression. Northern blot of testis RNA confirmed the absence of calspermin transcript. Northern blot of testis and thymus RNA showed that Camk4 kinase expression was unaffected. (J:70856)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Camk4 Mutation: 39 strains or lines available

References

• Original: J:70856 Wu JY, et al., Spermatogenesis and the regulation of Ca(2+)-calmodulin-dependent protein kinase IV localization are not dependent on calspermin. Mol Cell Biol. 2001 Sep;21(17):6066-70
• All: 1 reference(s)