Rag1^tm1(GFP)Imku
Targeted Allele Detail

Summary

• Symbol: Rag1^tm1(GFP)Imku
• Name: recombination activating 1; targeted mutation 1, Nobuo Sakaguchi
• MGI ID: MGI:2388344
• Synonyms: rag1/gfp, Rag1^gfp, RAG1-GFP
• Gene: Rag1 Location: Chr2:101468627-101479846 bp, - strand Genetic Position: Chr2, 53.88 cM
• Alliance: Rag1^tm1(GFP)Imku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79416
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The gene was disrupted by insertion (knock-in) of a GFP-neo cassette into the coding sequence. RT-PCR analysis of bone marrow RNA confirmed the absence of Rag1 gene expression in homozygous mutant animals. Instead the mutant animals produced GFP transcripts. (J:79416)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rag1 Mutation: 123 strains or lines available

References

• Original: J:79416 Kuwata N, et al., Cutting edge: absence of expression of RAG1 in peritoneal B-1 cells detected by knocking into RAG1 locus with green fluorescent protein gene. J Immunol. 1999 Dec 15;163(12):6355-9
• All: 46 reference(s)