Kirrel1^{Gt(VICTR20)20Lex}
Gene trapped Allele Detail

Summary

• Symbol: Kirrel1^{Gt(VICTR20)20Lex}
• Name: kirre like nephrin family adhesion molecule 1; gene trap 20, Lexicon Genetics
• MGI ID: MGI:2388348
• Synonyms: Neph1^-
• Gene: Kirrel1 Location: Chr3:86985900-87082054 bp, - strand Genetic Position: Chr3, 38.18 cM, cytoband F1
• Alliance: Kirrel1^{Gt(VICTR20)20Lex} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74230
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene trap vector consisting of LTR sites flanking a beta-geo cassette containing a splice acceptor and a PGK-puromycin resistance cassette containing a splice donor integrated into the intron immediately downstream of the first coding exon. Northern blot analysis of kidney RNA confirmed the absence of normal transcript in homozygous mutant mice. Expression of Beta-geo was detected and putatively driven by the endogenous promoter. While expression from the internal PGK promoter was detected in ES cells, allowing for the identification of the disrupted gene, it was undetected by both Northern blot and RT-PCR analyses of RNA derived from mice. (J:74230)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kirrel1 Mutation: 194 strains or lines available

References

• Original: J:74230 Donoviel DB, et al., Proteinuria and perinatal lethality in mice lacking NEPH1, a novel protein with homology to NEPHRIN. Mol Cell Biol. 2001 Jul;21(14):4829-36
• All: 2 reference(s)