Cux1^tm1Mbu
Targeted Allele Detail

Summary

• Symbol: Cux1^tm1Mbu
• Name: cut-like homeobox 1; targeted mutation 1, Meinrad Busslinger
• MGI ID: MGI:2388352
• Synonyms: CDP-lacZ, Cutl1^Z
• Gene: Cux1 Location: Chr5:136276989-136596344 bp, - strand Genetic Position: Chr5, 75.96 cM
• Alliance: Cux1^tm1Mbu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71407
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 22 was fused in-frame to a lacZ cassette and exon 23 was replaced with a neomycin resistance cassette via homologous recombination. The mutation results in deletion of the C-terminal Cut repeat 3 and homeodomain, which disrupts all CDP isoforms. The CASP isoform remains unaffected although it is transcribed at 6-fold lower levels compared to wild-type. Expression of the CDP-lacZ fusion protein was verified by Western blot analysis of mouse embyronic fibroblasts (MEFs) derived from homozygous mutants. The fusion protein lacks gene repression activity and is therefore a null allele. (J:71407)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cux1 Mutation: 112 strains or lines available

References

• Original: J:71407 Ellis T, et al., The transcriptional repressor CDP (Cutl1) is essential for epithelial cell differentiation of the lung and the hair follicle. Genes Dev. 2001 Sep 1;15(17):2307-19
• All: 3 reference(s)