Folh1^tm1Nle
Targeted Allele Detail

Summary

• Symbol: Folh1^tm1Nle
• Name: folate hydrolase 1; targeted mutation 1, Joseph H Neale
• MGI ID: MGI:2388359
• Synonyms: Folh1^-, Folh1^tm1ski, PMSA^-
• Gene: Folh1 Location: Chr7:86368185-86425151 bp, - strand Genetic Position: Chr7, 48.51 cM, cytoband D1-D2
• Alliance: Folh1^tm1Nle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79368
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The endogenous locus was disrupted by the insertion of a vector that replaced the 3' and 5' splice sites of exons 1 and 2, respectively, with sequence containing stop codons. The targeting vector also included a neo cassette that replaced the majority of intron 1. Neither transcript nor protein was detected by Northern or Western blot analyses of brain tissue obtained from homozygous mutant mice. (J:79368)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Folh1 Mutation: 50 strains or lines available

References

• Original: J:79368 Bacich DJ, et al., Deletion of the glutamate carboxypeptidase II gene in mice reveals a second enzyme activity that hydrolyzes N-acetylaspartylglutamate. J Neurochem. 2002 Oct;83(1):20-9
• All: 5 reference(s)