Hoxb4^tm2.1Nrm
Targeted Allele Detail

Summary

• Symbol: Hoxb4^tm2.1Nrm
• Name: homeobox B4; targeted mutation 2.1, Nancy R Manley
• MGI ID: MGI:2388369
• Synonyms: Hoxb4^loxP
• Gene: Hoxb4 Location: Chr11:96209093-96212464 bp, + strand Genetic Position: Chr11, 59.83 cM
• Alliance: Hoxb4^tm2.1Nrm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71389
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele is derived from Hoxb4^tm2Nrm. Embryos from heterozygous Hoxb4^tm2Nrm intercrosses were injected with a cre expression plasmid to remove the floxed neo cassette in the first coding exon. The deletion leaves behind a 78 bp insertion containing a loxP site in the first exon. The 78 bp insertion contains a stop codon in-frame with the Hoxb4 transcript. (J:71389)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxb4 Mutation: 20 strains or lines available

Notes

ES cell line = R1-45, a subline of R1.

References

• Original: J:71389 Manley NR, et al., Hoxb2 and hoxb4 act together to specify ventral body wall formation. Dev Biol. 2001 Sep 1;237(1):130-44
• All: 1 reference(s)