Tnc^tm1Msch
Targeted Allele Detail

Summary

• Symbol: Tnc^tm1Msch
• Name: tenascin C; targeted mutation 1, Melitta Schachner
• MGI ID: MGI:2388370
• Synonyms: tn-C -/-
• Gene: Tnc Location: Chr4:63878022-63965252 bp, - strand Genetic Position: Chr4, 34.06 cM
• Alliance: Tnc^tm1Msch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78340
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 was replaced with a single loxP site by homologous recombination followed by in vitro cre mediated excision. The deleted region encoded the signal peptide and the tenascin assembly domain. While normal transcript was undetected in homozygous mutant mice, weak expression of a transcript in which exon 1 was spliced to exon 3 was identified by Northern blot and RT-PCR analyses. Sequence analysis showed that the aberrant splicing introduced a frameshift mutation. Quantitative immunoblot analysis indicated that protein expression was limited to less than 0.05% of the wild-type level. Immunhistochemical analysis supported this finding. (J:78340)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnc Mutation: 135 strains or lines available

References

• Original: J:78340 Evers MR, et al., Impairment of L-type Ca2+ channel-dependent forms of hippocampal synaptic plasticity in mice deficient in the extracellular matrix glycoprotein tenascin-C. J Neurosci. 2002 Aug 15;22(16):7177-94
• All: 15 reference(s)