Scd1^tm1Ntam
Targeted Allele Detail

Summary

• Symbol: Scd1^tm1Ntam
• Name: stearoyl-Coenzyme A desaturase 1; targeted mutation 1, James M Ntambi
• MGI ID: MGI:2388371
• Synonyms: SCD1^-
• Gene: Scd1 Location: Chr19:44382889-44396148 bp, - strand Genetic Position: Chr19, 37.98 cM
• Alliance: Scd1^tm1Ntam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71350
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region of the gene (exons 1-6) was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot analysis of various tissue samples and by Western blot analysis of liver protein extracts. Enzyme activity was also undetectable in liver extracts from homozygous animals. (J:71350)

Expression

In Mice Carrying this Mutation:	4 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Scd1 Mutation: 24 strains or lines available

References

• Original: J:71350 Miyazaki M, et al., Targeted disruption of stearoyl-CoA desaturase1 gene in mice causes atrophy of sebaceous and meibomian glands and depletion of wax esters in the eyelid. J Nutr. 2001 Sep;131(9):2260-8
• All: 45 reference(s)