Rdh5^tm1Drie
Targeted Allele Detail

Summary

• Symbol: Rdh5^tm1Drie
• Name: retinol dehydrogenase 5; targeted mutation 1, Carola A G G Driessen
• MGI ID: MGI:2388375
• Synonyms: 11-cis-rdh-
• Gene: Rdh5 Location: Chr10:128749457-128755906 bp, - strand Genetic Position: Chr10, 77.19 cM
• Alliance: Rdh5^tm1Drie page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77619
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exons 1-3 with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was verified by RT-PCR analysis of total RNA from eyes and kidneys, and by Western blot analysis of eye protein extracts. (J:77619)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rdh5 Mutation: 11 strains or lines available

References

• Original: J:77619 Driessen CA, et al., Disruption of the 11-cis-retinol dehydrogenase gene leads to accumulation of cis-retinols and cis-retinyl esters. Mol Cell Biol. 2000 Jun;20(12):4275-87
• All: 8 reference(s)