Tpgs1^{Gt(ROSA22)Sor}
Gene trapped Allele Detail

Summary

• Symbol: Tpgs1^{Gt(ROSA22)Sor}
• Name: tubulin polyglutamylase complex subunit 1; gene trap ROSA22, Philippe Soriano
• MGI ID: MGI:2388468
• Synonyms: Gm16517^{Gt(ROSA)22Sor} Gm16517^{Gt(ROSA)22Sor} Gm16517^{Gt(ROSA)22Sor}, Gtrgeo22^{Gt(ROSABetageo)22Sor}, ROSA22
• Gene: Tpgs1 Location: Chr10:79505273-79511961 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Tpgs1^{Gt(ROSA22)Sor} page

Mutation origin

• Mutant Cell Line: FHCRC-GT-ROSA22
• Germline Transmission: Earliest citation of germline transmission: J:79292
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: ROSABetageo
• Mutation details: A ROSA Beta-geo gene trap vector randomly integrated into an intron between the two coding exons of the endogenous locus. Wild-type mRNA was undetected by RT-PCR analysis of total RNA obtained from homozygous mutant testes tissue. Transcript levels produced by adjacent genes, Madcam1 and Cdc34, were normal, indicating that they were unaffected by the insertion. The expression pattern of the integrated beta-galactosidase sequence was determined to be similar to that of the endogenous gene as determined by in situ hybridization. (J:79292)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  FHCRC-GT-ROSA22	CG465837 ; (MGI Seq Detail)	5' RACE	Chr10:79504995-79505616 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  184 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpgs1 Mutation: 87 strains or lines available

References

• Original: J:79292 Campbell PK, et al., Mutation of a novel gene results in abnormal development of spermatid flagella, loss of intermale aggression and reduced body fat in mice. Genetics. 2002 Sep;162(1):307-20
• All: 2 reference(s)