Il6st^tm1Ard
Targeted Allele Detail

Summary

• Symbol: Il6st^tm1Ard
• Name: interleukin 6 signal transducer; targeted mutation 1, Ashley R Dunn
• MGI ID: MGI:2388476
• Synonyms: gp130^deltaSTAT
• Gene: Il6st Location: Chr13:112600604-112643394 bp, + strand Genetic Position: Chr13, 63.73 cM
• Alliance: Il6st^tm1Ard page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79555
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: The 4 signal transducer and activator of transcription (STAT) sites in exon 18 were deleted by homologous recombination of a construct containing two missense mutations (disrupting the first site) and an IRES-neo cassette (replacing the other 3 sites). The missense mutations were at codons 765 (tyrosine to phenylalaine) and 768 (glutamine to alanine). A nonsense mutation was engineered at codon 769, immediately preceding the IRES-neo cassette. Transcription of the targeted allele was verified by Northern blot analysis. (J:79555)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Il6st Mutation: 80 strains or lines available

References

• Original: J:79555 Ernst M, et al., Defective gp130-mediated signal transducer and activator of transcription (STAT) signaling results in degenerative joint disease, gastrointestinal ulceration, and failure of uterine implantation. J Exp Med. 2001 Jul 16;194(2):189-203
• All: 18 reference(s)