Igh^tm1Cgn
Targeted Allele Detail

Summary

• Symbol: Igh^tm1Cgn
• Name: immunoglobulin heavy chain complex; targeted mutation 1, University of Cologne
• MGI ID: MGI:2388483
• Synonyms: IgH^T15i,a
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79557
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encompassing a 3' Igh-D element (DQ52), the Igh-J elements, and the Igh intron enhancer (Rr150) was replaced with a cassette containing a neomycin resistance gene followed by sequence encoding the variable region of a phosphorylcholine antibody. Flow cytometry verified cell surface expression on peripheral blood lymphocytes obtained from heterozygous and homozygous mice. (J:79557)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

Notes

The targeted IgH allele was of the a allotype.

References

• Original: J:79557 Taki S, et al., Targeted insertion of a variable region gene into the immunoglobulin heavy chain locus. Science. 1993 Nov 19;262(5137):1268-71
• All: 13 reference(s)