Igh^tm2Cgn
Targeted Allele Detail

Summary

• Symbol: Igh^tm2Cgn
• Name: immunoglobulin heavy chain complex; targeted mutation 2, University of Cologne
• MGI ID: MGI:2388486
• Synonyms: B18, B1-8i, Igh^B1-8, IgH, B1-8-HC, VHEmu^a
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79556
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Homologous recombination and subsequent in vitro cre-mediated excision resulted in the replacement of endogenous sequence encompassing a 3' Igh-D element (DQ52) and the Igh-J elements with a recombined antibody variable region derived from a 4-hydroxy-3-nitrophenyl acetyl binding antibody (B1-8). The B1-8 variable region was modified to contain a point mutation in codon 92, inactivating the heptameric recombination signal sequence. Flow cytometry verified cell surface expression of the mutant protein product. (J:79556)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

Notes

The targeted IgH allele was of the a allotype.

References

• Original: J:79556 Sonoda E, et al., B cell development under the condition of allelic inclusion. Immunity. 1997 Mar;6(3):225-33
• All: 82 reference(s)