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Tg(CD4-HIV)F21407Pjo
Transgene Detail
Summary
Symbol: Tg(CD4-HIV)F21407Pjo
Name: transgene insertion F21407, Paul Jolicoeur
MGI ID: MGI:2388592
Synonyms: CD4C/HIVMutA
Transgene: Tg(CD4-HIV)F21407Pjo  Location: unknown  
Alliance: Tg(CD4-HIV)F21407Pjo page
Transgene
origin
Strain of Origin:  (C57BL/6 x C3H)F2
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsMouse CD4 enhancer sequences, the human CD4 promoter, 8.8 kb of HIV sequences, and an SV40 polyadenylation signal was used for the transgene. The enhancer and promoter sequences were used to drive expression in CD4+ T cells and cells of the dendritic/macrophage lineage. The HIV sequence contained the following mutations: stop oligos (a 14-mer sequence introdicing stop codons in all 3 reading frames) in the gag and vif genes, a 22 bp deletion of the pol gene followed by a stop oligo, a deletion of nucleotides 5622-5737 in the vpr gene, a deletion of nucleotides 6062-6180 in the vpu gene, and a G to T nonsense mutation at nucleotide5854 of the tat gene. Northern blot analysis showed high transgene expression in the thymus, moderate expression in lymph nodes, and weak expression in kidneys, lungs, intestines, and liver. (J:78756)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Notes
Transgenic founder lines F21380, F21407, and F21388 were established. The latency of disease if reflective of the level of transgene expression. Founders F21388 and F21407 express high levels of the transgene compared to founder F21380. Survival time is the shortest in founder F21407, intermediate in founder F21388, and longest in founder F21380.
References
Original:  J:78756 Hanna Z, et al., Nef harbors a major determinant of pathogenicity for an AIDS-like disease induced by HIV-1 in transgenic mice. Cell. 1998 Oct 16;95(2):163-75
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory