Psme1/Psme2^tm1Tchi
Targeted Allele Detail

Summary

• Symbol: Psme1/Psme2^tm1Tchi
• Name: proteasome (prosome, macropain) activator subunit 2 (PA28 beta); targeted mutation 1, Tomoki Chiba
• MGI ID: MGI:2388601
• Synonyms: PA28alpha^-beta^-
• Gene: Psme2 Location: Chr14:55824897-55828558 bp, - strand Genetic Position: Chr14, 28.19 cM, cytoband C2-D1
• Alliance: Psme1/Psme2^tm1Tchi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72533
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct where exons 2-10 of Psme1 were replaced with a neomycin resistant cassette and exons 2-10 of Psme2 were deleted was used to disrupt both loci in a single homologous recombination event. The sequence of Cgi112 (1500005A01Rik), the gene located between Psme1 and Psme2, remained unchanged. Northern blot and Western blot analysis of mouse embryonic fibroblast (MEFs) cultured from homozygous mutant embryos confirmed the absence of Psme1 and Psme2 gene expression. (J:72533)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Psme2 Mutation: 17 strains or lines available

Notes

Homozygous mutant animals breed well and appear healthy with no observable abnormalities.

References

• Original: J:72533 Murata S, et al., Immunoproteasome assembly and antigen presentation in mice lacking both PA28alpha and PA28beta. EMBO J. 2001 Nov 1;20(21):5898-907
• All: 12 reference(s)