Tcrg^tm2.1Dhr
Targeted Allele Detail

Summary

• Symbol: Tcrg^tm2.1Dhr
• Name: T cell receptor gamma chain; targeted mutation 2.1, David H Raulet
• MGI ID: MGI:2388612
• Synonyms: H/E^-, Trgc1^tm1.1Dhr
• Gene: Tcrg Location: Chr13:19362212-19540646 bp, + strand Genetic Position: Chr13, cytoband A2-A3

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75624
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Embryonic stem cells carrying a deletion for the HsA enhancer element (Rr282726) were subjected to gene targeting to replace the Ecgamma 1 enhancer element (Rr275254) with a loxP site flanked neomycin resistance gene cassette. Correctly targeted cells, in which the Ecgamma 1 enhancer element was mutated on the same chromosome as the HsA deletion, were transfected with a cre expression plasmid to remove the floxed neo cassette. (J:75624)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrg Mutation: 1 strain or line available

References

• Original: J:75624 Xiong N, et al., Redundant and unique roles of two enhancer elements in the TCRgamma locus in gene regulation and gammadelta T cell development. Immunity. 2002 Mar;16(3):453-63
• All: 1 reference(s)