Grpr^tm1Wada
Targeted Allele Detail

Summary

• Symbol: Grpr^tm1Wada
• Name: gastrin releasing peptide receptor; targeted mutation 1, Keiji Wada
• MGI ID: MGI:2388628
• Gene: Grpr Location: ChrX:162296900-162332673 bp, - strand Genetic Position: ChrX, 75.63 cM
• Alliance: Grpr^tm1Wada page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43565
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 5' portion of exon 2 was deleted by the insertion of a neomycin selection cassette. RT-PCR analysis demonstrated the absence of normal transcript in mutant mice. Functional ablation was demonstrated by monitoring body temperature after the administration of gastrin releasing peptide (Grp). A ligand binding assay further confirmed the absence of functional protein. (J:43565)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grpr Mutation: 14 strains or lines available

References

• Original: J:43565 Wada E, et al., Generation and characterization of mice lacking gastrin-releasing peptide receptor. Biochem Biophys Res Commun. 1997 Oct 9;239(1):28-33
• All: 11 reference(s)