Oprm1^tm1Loh
Targeted Allele Detail

Summary

• Symbol: Oprm1^tm1Loh
• Name: opioid receptor, mu 1; targeted mutation 1, Horace H Loh
• MGI ID: MGI:2388673
• Synonyms: MOP-KO, MORKO
• Gene: Oprm1 Location: Chr10:6708593-6988209 bp, + strand Genetic Position: Chr10, 1.85 cM, cytoband A2
• Alliance: Oprm1^tm1Loh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47187
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 where replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded six of the seven transmembrane domains. Ligand binding assays demonstrated the absence of a functional protein product. Phenotypic observations further indicated the ablation of both receptor subtypes. (J:47187)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Oprm1 Mutation: 54 strains or lines available

References

• Original: J:47187 Loh HH, et al., mu Opioid receptor knockout in mice: effects on ligand-induced analgesia and morphine lethality. Brain Res Mol Brain Res. 1998 Mar 1;54(2):321-6
• All: 39 reference(s)