Trp53^tm2Mok
Targeted Allele Detail

Summary

• Symbol: Trp53^tm2Mok
• Name: transformation related protein 53; targeted mutation 2, Motoya Katsuki
• MGI ID: MGI:2388703
• Synonyms: p53^-, Trp53^tm1.1Mok
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm2Mok page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79629
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted using a two-step targeting strategy. First, exons 2-10 were replaced with a neo-TK cassette via homologous recombination. Next, correctly targeted ES cells were subjected to another round of gene targeting to replace the neo-TK cassette with a lacZ cassette. Successful gene replacement was selected for via gancyclovir resistance. Homozygous mutant animals were successfully established. (J:79629)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm2Mok

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:79629 Gondo Y, et al., Gene replacement of the p53 gene with the lacZ gene in mouse embryonic stem cells and mice by using two steps of homologous recombination. Biochem Biophys Res Commun. 1994 Jul 29;202(2):830-7
• All: 9 reference(s)